Electrochemical impedance biosensor based on Y chromosome-specific sequences for fetal sex determination.

A new electrochemical biosensor based on the sequence of chromosome Y (SRY) has been introduced to determine the gender of the fetus. At first, the DNA probe was designed based on the SRY gene sequence on chromosome Y. Then, a suitable functional group was added to the DNA probe, and it has been immobilized on the surface of the electrode modified with a nanocomposite containing Cu(OH)2 @N-C n-boxes. This substrate causes more DNA probes to connect to the electrode surface by increasing the effective surface area. The presence of the SRY sequence in the DNA sample extracted from blood was detected by the electrochemical signal of the bio-sensor. After optimizing the parameters, the fabricated genosensor showed linear responses in the two concentration ranges containing 0.5 fM to 50 pM and 50 pM to 500 nM. The limit of detection (LOD) for the proposed method was 0.16 fM. The proposed genosensor has been successfully used to determine the gender of the fetus using cell-free fetal DNA (cffDNA) in the blood plasma of several pregnant mothers. This method has advantages such as being simple, portable, accurate, and non-invasive for early determination of the gender of the fetus and early diagnosis of X-linked genetic disorders.

Fabrication of an electrochemical aptasensor for the determination of sarcosine based on synthesized CuCo2O4 nanosheets.

Sarcosine (SRN) detection in body fluids is related to the diagnosis of prostate cancer. However, the development of SRN biosensors has been limited due to the low concentration of SRN in body fluids. Here, a new electrochemical strategy for selective and accurate determination of SRN in urine samples is reported. CuCo2O4 nanosheets (CuCo2O4 NSs) have been synthesized and used as a new platform in the design of efficient electrochemical aptasensors for prostate cancer diagnosis. As far as we know, CuCo2O4 NSs have not been used so far in electrochemical aptasensor design. The presence of CuCo2O4 NSs on the electrode surface as a platform improves the conductivity and surface area. Therefore, it can be very effective in improving the diagnostic performance of the electrochemical aptasensor. The linear concentration range and limit of detection (LOD) for this strategy were calculated to be 1 pM- 8 µM and 350 fM, respectively.

Flower-like core-shell nanostructures based on natural asphalt coated with Ni-LDH nanosheets as an electrochemical platform for prostate cancer biomarker sensing.

Flower-like core-shell nanostructures based on natural asphalt (NA) coated with nickel-layered double hydroxide nanosheets (Ni-LDH NSs) were synthesized for the first time. The synthetic nanostructures were successfully used as an efficient platform in the design of sarcosine (SAR) electrochemical aptasensor. SAR is considered an efficient biomarker for prostate cancer (PCa) diagnosis. However, the low concentration of SAR in urine, plasma, and tissue cells has limited the growth of SAR biosensors. The performance of the presented SAR aptasensor is very promising in being applied as a portable device in the identification of PCa. After drawing the calibration curve, the linear concentration range was obtained in two ranges from 5 pM to 100 nM and 100 nM to 7.9 µM, and the limit of detection (LOD) was calculated to be 1.6 pM. This study can provide a basis for wider research in various programs such as developing PCa diagnostic aptasensors and investigating the use of NA nanostructures in other electrochemical applications such as electrocatalysis and energy storage.

The development of an electrochemical nanoaptasensor to sensing chloramphenicol using a nanocomposite consisting of graphene oxide functionalized with (3-Aminopropyl) triethoxysilane and silver nanoparticles.

In the present research, a nanoaptasensor is proposed for electrochemical measurement of chloramphenicol (CAP). To this purpose, the nanocomposite prepared from graphene oxide and functionalized with (3-Aminopropyl) triethoxysilane/silver nanoparticles to the abbreviated AgNPs/[NH2-Si]-f-GO, was utilized to modify the glassy carbon electrode (GCE). Furthermore, the modified electrode was also investigated using the electrochemical methods such as electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The AgNPs/[NH2-Si]-f-GO nanocomposite was investigated by UV-Vis spectrophotometry. Fourier transform infrared (FT-IR) spectrometry and transmission electron microscopy (TEM). Moreover, [Fe(CN)6]3-/4 solution in the role of an electrochemical probe was applied. The AgNPs/[NH2-Si]-f-GO nanocomposite was confirmed as a good layer to covalent immobilization of aptamer (Apt) onto the GCE surface. In this sense, the DPV was used as a sensitive electrochemical technique for the measurement of CAP with an appropriate linear concentration range which was found to be between 10 pM and 0.2 µM and, with a low limit of detection, it equaled 3.3 pM. CAP which was identified in the presence of other usual antibiotics existed in the real samples.